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KD-Validated Anti-PSMD4 Rabbit Monoclonal Antibody #61699

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Flow cytometric analysis of PSMD4 expression in HepG2 cells using anti-PSMD4 antibody (Cat#61699, 1:2,000). Green, isotype control; red, PSMD4.
Western blotting analysis using anti-PSMD4 antibody (Cat#61699). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMD4 antibody (Cat#61699, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PSMD4 antibody (Cat#61699). PSMD4 expression in wild type (WT) and PSMD4 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PSMD4 antibody (Cat#61699, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    PSMD4
  • 货号:
    61699
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-PSMD4 Rabbit Monoclonal Antibody
Aliases PSMD4; Proteasome 26S Subunit Ubiquitin Receptor, Non-ATPase 4; AF; Rpn10; AF-1; S5A; Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4; 26S Proteasome Non-ATPase Regulatory Subunit 4; 26S Proteasome Regulatory Subunit S5A; Proteasome 26S Subunit, Non-ATPase 4; Multiubiquitin Chain-Binding Protein; Antisecretory Factor 1; MCB1; ASF; 26S Proteasome Regulatory Subunit RPN10; S5a/Antisecretory Factor Protein; Angiocidin; PUB-R5
Background

Gene Name: PSMD4

NCBI Gene Entry: 5710

UniProt Entry: P55036

Application Information

Molecular Weight: Predicted, 41 kDa, observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3365

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PSMD4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PSMD4 expression in HepG2 cells using anti-PSMD4 antibody (Cat#61699, 1:2,000). Green, isotype control; red, PSMD4.
  • Western blotting analysis using anti-PSMD4 antibody (Cat#61699). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMD4 antibody (Cat#61699, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-PSMD4 antibody (Cat#61699). PSMD4 expression in wild type (WT) and PSMD4 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PSMD4 antibody (Cat#61699, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of PSMD4 expression in HepG2 cells using anti-PSMD4 antibody (Cat#61699, 1:2,000). Green, isotype control; red, PSMD4.
Western blotting analysis using anti-PSMD4 antibody (Cat#61699). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMD4 antibody (Cat#61699, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PSMD4 antibody (Cat#61699). PSMD4 expression in wild type (WT) and PSMD4 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PSMD4 antibody (Cat#61699, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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