Flow cytometric analysis of Paraoxonase 2 expression in HepG2 cells using anti-Paraoxonase 2 antibody (Cat#62478, 1:2,000). Green, isotype control; red, Paraoxonase 2.
Immunocytochemical staining of HepG2 cells with anti-Paraoxonase 2 antibody(Cat#62478, 1:1,000) . Nuclei were stained blue with DAPI; Paraoxonase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Paraoxonase 2 antibody (Cat#62478). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Paraoxonase 2 antibody (Cat#62478, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Paraoxonase 2 antibody (Cat#62478). Paraoxonase 2 expression in wild type (WT) and Paraoxonase 2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Paraoxonase 2 antibody (Cat#62478, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
