Flow cytometric analysis of Myosin XIX expression in HepG2 cells using anti-Myosin XIX antibody (Cat#61708, 1:2,000). Green, isotype control; red, Myosin XIX.
Immunocytochemical staining of HepG2 cells with anti-Myosin XIX antibody (Cat#61708, 1:1,000). Nuclei were stained blue with DAPI; Myosin XIX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-myosin XIX antibody (Cat#61708). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-myosin XIX antibody (Cat#61708, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-myosin XIX antibody (Cat#61708). Myosin XIX expression in wild type (WT) and myosin XIX (MYO19) knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-myosin XIX antibody (Cat#61708, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
