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KD-Validated Anti-ANAPC2 Mouse Monoclonal Antibody #61785

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Flow cytometric analysis of Anaphase promoting complex subunit 2 expression in C2C12 cells using anti-Anaphase promoting complex subunit 2 antibody (Cat#61785, 1:2,000). Green, isotype control; red, Anaphase promoting complex subunit 2.

Western blotting analysis using anti-anaphase promoting complex subunit 2 antibody (Cat#61785). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-anaphase promoting complex subunit 2 antibody (Cat#61785, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-anaphase promoting complex subunit 2 antibody (Cat#61785). Anaphase promoting complex subunit 2 expression in wild type (WT) and anaphase promoting complex subunit 2 (ANAPC2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-anaphase promoting complex subunit 2 antibody (Cat#61785, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ANAPC2
货号：

61785
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-ANAPC2 Mouse Monoclonal Antibody
Aliases	ANAPC2; Anaphase Promoting Complex Subunit 2; APC2; KIAA1406; Anaphase-Promoting Complex Subunit 2; Cyclosome Subunit 2
Background	Gene Name: ANAPC2 NCBI Gene Entry: 29882 UniProt Entry: Q9UJX6
Application Information	Molecular Weight: Predicted, 94 kDa; observed, 100 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB3750 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human ANAPC2
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Anaphase promoting complex subunit 2 expression in C2C12 cells using anti-Anaphase promoting complex subunit 2 antibody (Cat#61785, 1:2,000). Green, isotype control; red, Anaphase promoting complex subunit 2.
Western blotting analysis using anti-anaphase promoting complex subunit 2 antibody (Cat#61785). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-anaphase promoting complex subunit 2 antibody (Cat#61785, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-anaphase promoting complex subunit 2 antibody (Cat#61785). Anaphase promoting complex subunit 2 expression in wild type (WT) and anaphase promoting complex subunit 2 (ANAPC2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-anaphase promoting complex subunit 2 antibody (Cat#61785, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ANAPC2
货号：

61785
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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