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KD-Validated Anti-ALG2 Mouse Monoclonal Antibody #61787

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Western blotting analysis using anti-ALG2 antibody (Cat#61787). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALG2 antibody (Cat#61787, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ALG2 antibody (Cat#61787). ALG2 expression in wild-type (WT) and ALG2 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALG2 antibody (Cat#61787, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-ALG2 antibody (Cat#61787, 1:1000) . Nuclei were stained blue with DAPI; ALG2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
  • 基因名：
    ALG2
  • 货号：
    61787
  • 应用：
    WB
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-ALG2 Mouse Monoclonal Antibody
Aliases ALG2; ALG2 Alpha-1,3/1,6-Mannosyltransferase; HALPG2; CDGIi; NET38; CDG1I; Asparagine-Linked Glycosylation 2 Homolog (Yeast, Alpha-1,3-Mannosyltransferase); GDP-Man:Man(1)GlcNAc(2)-PP-Dol Alpha-1,3-Mannosyltransferase; GDP-Man:Man(2)GlcNAc(2)-PP-Dol Alpha-1,6-Mannosyltransferase; GDP-Man:Man(1)GlcNAc(2)-PP-Dolichol Mannosyltransferase; Asparagine-Linked Glycosylation Protein 2 Homolog; Alpha-1,3/1,6-Mannosyltransferase ALG2; FLJ14511; Asparagine-Linked Glycosylation 2, Alpha-1,3-Mannosyltransferase Homolog (S. Cerevisiae); Asparagine-Linked Glycosylation 2 Homolog (S. Cerevisiae, Alpha-1,3-Mannosyltransferase); Asparagine-Linked Glycosylation 2, Alpha-1,3-Mannosyltransferase Homolog; Alpha-1,3-Mannosyltransferase ALG2; Homolog Of Yeast ALG2; EC 2.4.1.132; EC 2.4.1.257; EC 2.4.1; CMSTA3; CMS14
Background

NCBI Gene Entry: 85365

UniProt Entry: Q9H553
Application Information

Molecular Weight: Predicted MW:47 kDa Observed MW:49 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3760

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen Recombinant protein of human ALG2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-ALG2 antibody (Cat#61787). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALG2 antibody (Cat#61787, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-ALG2 antibody (Cat#61787). ALG2 expression in wild-type (WT) and ALG2 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALG2 antibody (Cat#61787, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with anti-ALG2 antibody (Cat#61787, 1:1000) . Nuclei were stained blue with DAPI; ALG2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    ALG2
  • 货号：
    61787
  • 应用：
    WB
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-ALG2 antibody (Cat#61787). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALG2 antibody (Cat#61787, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ALG2 antibody (Cat#61787). ALG2 expression in wild-type (WT) and ALG2 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALG2 antibody (Cat#61787, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-ALG2 antibody (Cat#61787, 1:1000) . Nuclei were stained blue with DAPI; ALG2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
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