Flow cytometric analysis of Suppressor of cytokine signaling 2 expression in HAP-1 cells using anti-Suppressor of cytokine signaling 2 antibody (Cat#3356, 1:2,000). Green, isotype control; red, Suppressor of cytokine signaling 2.
Immunocytochemical staining of HAP-1 with anti-suppressor of cytokine signaling 2 antibody (Cat#3356, 1:1,000). Nuclei were stained blue with DAPI; Suppressor of cytokine signaling 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-suppressor of cytokine signaling 2 antibody (Cat#3356). Total cell lysates (10 μg for HAP1 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-suppressor of cytokine signaling 2 antibody (Cat#3356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Suppressor of cytokine signaling 2 expression in HAP-1 cells using anti-Suppressor of cytokine signaling 2 antibody (Cat#3356, 1:2,000). Green, isotype control; red, Suppressor of cytokine signaling 2.
Immunocytochemical staining of HAP-1 with anti-suppressor of cytokine signaling 2 antibody (Cat#3356, 1:1,000). Nuclei were stained blue with DAPI; Suppressor of cytokine signaling 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-suppressor of cytokine signaling 2 antibody (Cat#3356). Total cell lysates (10 μg for HAP1 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-suppressor of cytokine signaling 2 antibody (Cat#3356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Suppressor of cytokine signaling 2 expression in HAP-1 cells using anti-Suppressor of cytokine signaling 2 antibody (Cat#3356, 1:2,000). Green, isotype control; red, Suppressor of cytokine signaling 2.
Immunocytochemical staining of HAP-1 with anti-suppressor of cytokine signaling 2 antibody (Cat#3356, 1:1,000). Nuclei were stained blue with DAPI; Suppressor of cytokine signaling 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-suppressor of cytokine signaling 2 antibody (Cat#3356). Total cell lysates (10 μg for HAP1 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-suppressor of cytokine signaling 2 antibody (Cat#3356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
