Flow cytometric analysis of Mitogen-activated protein kinase 9 expression in HeLa cells using anti-Mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,000). Green, isotype control; red, Mitogen-activated protein kinase 9.
Western blotting analysis using anti-mitogen-activated protein kinase 9 antibody (Cat#61790). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-mitogen-activated protein kinase 9 antibody (Cat#61790). Mitogen-activated protein kinase 9 expression in wild-type (WT) and mitogen-activated protein kinase 9 (MAPK9) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-mitogen-activated protein kinase 9 antibody (Cat#61790, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).