Flow cytometric analysis of APMAP expression in HT-1080 cells using anti-APMAP antibody (Cat#5342, 1:2,000). Green, isotype control; red, APMAP.
Immunocytochemical staining of HT-1080 cells with anti-APMAP antibody (Cat#5342, 1:1,000). Nuclei were stained blue with DAPI; APMAP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-APMAP antibody (Cat#5342). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APMAP antibody (Cat#5342, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).