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KD-Validated Anti-Dihydropyrimidinase Like 5 Mouse Monoclonal Antibody #61833

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Flow cytometric analysis of Dihydropyrimidinase like 5 expression in HAP-1 cells using anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,000). Green, isotype control; red, Dihydropyrimidinase like 5.
Immunocytochemical staining of HAP1 cells with anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:1,000). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-dihydropyrimidinase like 5 antibody (Cat#61833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-dihydropyrimidinase like 5 antibody (Cat#61833). Dihydropyrimidinase like 5 expression in wild-type (WT) and dihydropyrimidinase like 5 (DPYSL5) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-dihydropyrimidinase like 5 antibody (Cat#61833, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Dihydropyrimidinase like 5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HSHC cells using anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:1,000), Top panel: wild-type (WT); Bottom panal: Dihydropyrimidinase like 5 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名:
    DPYSL5
  • 货号:
    61833
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Dihydropyrimidinase Like 5 Mouse Monoclonal Antibody
Aliases DPYSL5; Dihydropyrimidinase Like 5; CRMP-5; CRMP5; CRAM; Ulip6; CV2; Collapsin Response Mediator Protein 5; Dihydropyrimidinase-Related Protein 5; UNC33-Like Phosphoprotein 6; CRMP3-Associated Molecule; ULIP-6; DRP-5; Collapsin Response Mediator Protein-5; Dihydropyrimidinase-Like 5; RTSC4; ULIP6
Background

Gene Name: DPYSL5

NCBI Gene Entry: 56896

UniProt Entry: Q9BPU6

Application Information

Molecular Weight: Predicted, 61 kDa; observed, 61 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3935

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human CRMP-5
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Dihydropyrimidinase like 5 expression in HAP-1 cells using anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,000). Green, isotype control; red, Dihydropyrimidinase like 5.
  • Immunocytochemical staining of HAP1 cells with anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:1,000). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-dihydropyrimidinase like 5 antibody (Cat#61833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-dihydropyrimidinase like 5 antibody (Cat#61833). Dihydropyrimidinase like 5 expression in wild-type (WT) and dihydropyrimidinase like 5 (DPYSL5) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-dihydropyrimidinase like 5 antibody (Cat#61833, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Dihydropyrimidinase like 5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HSHC cells using anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:1,000), Top panel: wild-type (WT); Bottom panal: Dihydropyrimidinase like 5 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
Flow cytometric analysis of Dihydropyrimidinase like 5 expression in HAP-1 cells using anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,000). Green, isotype control; red, Dihydropyrimidinase like 5.
Immunocytochemical staining of HAP1 cells with anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:1,000). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-dihydropyrimidinase like 5 antibody (Cat#61833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-dihydropyrimidinase like 5 antibody (Cat#61833). Dihydropyrimidinase like 5 expression in wild-type (WT) and dihydropyrimidinase like 5 (DPYSL5) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-dihydropyrimidinase like 5 antibody (Cat#61833, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Dihydropyrimidinase like 5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HSHC cells using anti-Dihydropyrimidinase like 5 antibody (Cat#61833, 1:1,000), Top panel: wild-type (WT); Bottom panal: Dihydropyrimidinase like 5 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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