Flow cytometric analysis of C17orf58 expression in HT-1080 cells using anti-C17orf58 antibody (Cat#61840, 1:1,000). Green, isotype control; red, C17orf58.
Immunocytochemical staining of HT-1080 cells with anti-C17orf58 associated protein antibody (Cat#61840, 1:1,000). Nuclei were stained blue with DAPI; C17orf58 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-c17orf58 antibody (Cat#61840). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-c17orf58 antibody (Cat#61840, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-C17orf58 antibody (Cat#61840). C17orf58 expression in wild type (WT) and C17orf58 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-C17orf58 antibody (Cat#61840, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
