Flow cytometric analysis of Dystrobrevin alpha expression in C2C12 cells using anti-Dystrobrevin alpha antibody (Cat#61845, 1:1,000). Green, isotype control; red, Dystrobrevin alpha.
Immunocytochemical staining of C2C12 cells with anti-Dystrobrevin alpha antibody (Cat#61845, 1:1,000). Nuclei were stained blue with DAPI; Dystrobrevin alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-dystrobrevin alpha antibody (Cat#61845). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dystrobrevin alpha antibody (Cat#61845, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-dystrobrevin alpha antibody (Cat#61845). Dystrobrevin alpha expression in wild type (WT) and dystrobrevin alpha (DTNA) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-dystrobrevin alpha antibody (Cat#61845, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
