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KD-Validated Anti-Phospho-PBK/TOPK (Thr9) Rabbit Monoclonal Antibody #61861

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Flow cytometric analysis of Phospho-PBK/TOPK (Thr9) expression in HepG2 cells using Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:2,000). Green, isotype control; red, Phospho-PBK/TOPK (Thr9).
Immunocytochemical staining of Hela cells with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PBK/TOPK (Thr9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861). Phospho-PBK/TOPK (Thr9) expression in wild type (WT) and Phospho-PBK/TOPK (Thr9) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-PBK/TOPK (Thr9) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-PBK/TOPK (Thr9) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    PBK
  • 货号：
    61861
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-PBK/TOPK (Thr9) Rabbit Monoclonal Antibody
Aliases PBK; PDZ Binding Kinase; TOPK; CT84; SPK; T-LAK Cell-Originated Protein Kinase; Cancer/Testis Antigen 84; Nori-3; Lymphokine-Activated Killer T-Cell-Originated Protein Kinase; Spermatogenesis-Related Protein Kinase; MAPKK-Like Protein Kinase; FLJ14385; Serine/Threonine Protein Kinase; Epididymis Luminal Protein 164; PDZ-Binding Kinase; EC 2.7.12.2; HEL164; NORI-3
Background

Gene Name: PBK

NCBI Gene Entry: 55872

UniProt Entry: Q96KB5
Application Information

Molecular Weight: Predicted, 36 kDa, observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4910

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-PBK/TOPK (Thr9)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-PBK/TOPK (Thr9) expression in HepG2 cells using Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:2,000). Green, isotype control; red, Phospho-PBK/TOPK (Thr9).
  • Immunocytochemical staining of Hela cells with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PBK/TOPK (Thr9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861). Phospho-PBK/TOPK (Thr9) expression in wild type (WT) and Phospho-PBK/TOPK (Thr9) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-PBK/TOPK (Thr9) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-PBK/TOPK (Thr9) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    PBK
  • 货号：
    61861
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-PBK/TOPK (Thr9) expression in HepG2 cells using Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:2,000). Green, isotype control; red, Phospho-PBK/TOPK (Thr9).
Immunocytochemical staining of Hela cells with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PBK/TOPK (Thr9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861). Phospho-PBK/TOPK (Thr9) expression in wild type (WT) and Phospho-PBK/TOPK (Thr9) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#61861, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-PBK/TOPK (Thr9) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-PBK/TOPK (Thr9) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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