Western blotting analysis using anti-WTAP antibody (Cat#61884). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WTAP antibody (Cat#61884, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-WTAP antibody (Cat#61884). WTAP expression in wild-type (WT) and WTAP shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-WTAP antibody (Cat#61884, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of WTAP expression in HeLa cells using anti-WTAP antibody (Cat#61884, 1：2000). Green, isotype control; red, WTAP.
Immunocytochemical staining of Hela cells with anti-WTAP antibody (Cat#61884, 1:1000) . Nuclei were stained blue with DAPI; WTAP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
