Flow cytometric analysis of ZNF598 expression in HT-1080 cells using anti-ZNF598 antibody (Cat#61910, 1:2,000). Green, isotype control; red, ZNF598.
Immunocytochemical staining of HT-1080 cells with anti-ZNF598 antibody (Cat#61910, 1:1,000). Nuclei were stained blue with DAPI; ZNF598 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-ZNF598 antibody (Cat#61910). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZNF598 antibody (Cat#61910, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ZNF598 antibody (Cat#61910). ZNF598 expression in wild-type (WT) and ZNF598 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ZNF598 antibody (Cat#61910, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
