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KO-Validated Anti-Chromobox 3 Mouse Monoclonal Antibody #71261

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Western blotting analysis using anti-chromobox 3 antibody (Cat#71261). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-chromobox 3 antibody (Cat#71261, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-chromobox 3 antibody (Cat#71261). Chromobox 3 expression in wild-type (WT) and chromobox 3 (CBX3) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-chromobox 3 antibody (Cat#71261, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CBX3
  • 货号:
    71261
  • 应用:
    WB
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-Chromobox 3 Mouse Monoclonal Antibody
Aliases CBX3; Chromobox 3; HP1Hs-Gamma; HP1gamma; Heterochromatin Protein 1 Homolog Gamma; Chromobox Protein Homolog 3; Modifier 2 Protein; HECH; Chromobox Homolog 3 (HP1 Gamma Homolog, Drosophila); Chromobox Homolog 3 (Drosophila HP1 Gamma); Heterochromatin Protein HP1 Gamma; HP1 Gamma Homolog (Drosophila); Heterochromatin-Like Protein 1; Chromobox Homolog 3; HP1 Gamma Homolog; HP1-GAMMA; HP1 Gamma
Background

NCBI Gene Entry: 11335

UniProt Entry: Q13185

Application Information

Molecular Weight: Predicted MW:21 kDa Observed MW:20 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3950

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human HP1 gamma
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-chromobox 3 antibody (Cat#71261). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-chromobox 3 antibody (Cat#71261, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-chromobox 3 antibody (Cat#71261). Chromobox 3 expression in wild-type (WT) and chromobox 3 (CBX3) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-chromobox 3 antibody (Cat#71261, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-chromobox 3 antibody (Cat#71261). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-chromobox 3 antibody (Cat#71261, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-chromobox 3 antibody (Cat#71261). Chromobox 3 expression in wild-type (WT) and chromobox 3 (CBX3) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-chromobox 3 antibody (Cat#71261, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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