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KD-Validated Anti-NR2C2 Rabbit Monoclonal Antibody #62414

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Flow cytometric analysis of NR2C2 expression in HepG2 cells using NR2C2 antibody (Cat#62414, 1:2,000). Green, isotype control; red, NR2C2.
Immunocytochemical staining of HepG2 cells with anti-NR2C2 antibody (Cat#62414, 1:1,000). Nuclei were stained blue with DAPI; NR2C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NR2C2 antibody (Cat#62414). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NR2C2 antibody (Cat#62414, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NR2C2 antibody (Cat#62414). NR2C2 expression in wild-type (WT) and NR2C2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NR2C2 antibody (Cat#62414, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of NR2C2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-NR2C2 antibody (Cat#62414, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名:
    NR2C2
  • 货号:
    62414
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-NR2C2 Rabbit Monoclonal Antibody
Aliases Nuclear Receptor Subfamily 2 Group C Member 2; TAK1; TR4; Orphan Nuclear Receptor TAK1; Orphan Nuclear Receptor TR4; TR2R1; HTAK1; Nuclear Receptor Subfamily 2, Group C, Member 2; Testicular Nuclear Receptor 4; Nuclear Hormone Receptor TR4; Testicular Receptor 4; Orphan Receptor TR4
Background

Gene Name: NR2C2

NCBI Gene Entry: 7182

UniProt Entry: P49116

Application Information

Molecular Weight: Predicted, 65 kDa, observed, 65 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB730

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human NR2C2 / TR4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NR2C2 expression in HepG2 cells using NR2C2 antibody (Cat#62414, 1:2,000). Green, isotype control; red, NR2C2.
  • Immunocytochemical staining of HepG2 cells with anti-NR2C2 antibody (Cat#62414, 1:1,000). Nuclei were stained blue with DAPI; NR2C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-NR2C2 antibody (Cat#62414). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NR2C2 antibody (Cat#62414, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-NR2C2 antibody (Cat#62414). NR2C2 expression in wild-type (WT) and NR2C2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NR2C2 antibody (Cat#62414, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of NR2C2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-NR2C2 antibody (Cat#62414, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
Flow cytometric analysis of NR2C2 expression in HepG2 cells using NR2C2 antibody (Cat#62414, 1:2,000). Green, isotype control; red, NR2C2.
Immunocytochemical staining of HepG2 cells with anti-NR2C2 antibody (Cat#62414, 1:1,000). Nuclei were stained blue with DAPI; NR2C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NR2C2 antibody (Cat#62414). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NR2C2 antibody (Cat#62414, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NR2C2 antibody (Cat#62414). NR2C2 expression in wild-type (WT) and NR2C2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NR2C2 antibody (Cat#62414, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of NR2C2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-NR2C2 antibody (Cat#62414, 1:2,000) and analyzed using BD flow cytometer.
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