Immunocytochemical analysis of GPR171 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blotting analysis of GPR171 expression in HEK293T (A), Hela (B), H460 (C), mouse kidney (D), rat kidney (E), mouse lung (F) whole cell lysates.
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human GPR171
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000 Immunocytochemistry (IC): 1:50-1:200
Note
This product is for research use only.
Picture
Immunocytochemical analysis of GPR171 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blotting analysis of GPR171 expression in HEK293T (A), Hela (B), H460 (C), mouse kidney (D), rat kidney (E), mouse lung (F) whole cell lysates.
Immunocytochemical analysis of GPR171 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blotting analysis of GPR171 expression in HEK293T (A), Hela (B), H460 (C), mouse kidney (D), rat kidney (E), mouse lung (F) whole cell lysates.
