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KD-Validated Anti-Phospho-Rad17 (S656) Rabbit Monoclonal Antibody #62429

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Flow cytometric analysis of Phospho-Rad17 (S656) expression in HAP-1 cells using anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000). Green, isotype control; red, Phospho-Rad17 (S656).
Immunocytochemical staining of HAP-1 cells with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-Rad17 (S656) antibody (Cat#62429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-Rad17 (S656) antibody (Cat#62429). Phospho-Rad17 (S656) expression in wild-type (WT) and RAD17 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Rad17 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-Rad17 (S656) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    RAD17
  • 货号：
    62429
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-Rad17 (S656) Rabbit Monoclonal Antibody
Aliases RAD17 Checkpoint Clamp Loader Component; RAD17Sp; Rad24; CCYC; Cell Cycle Checkpoint Protein RAD17; R24L; Cell Cycle Checkpoint Protein (RAD17); RAD17 Homolog (S. Pombe); RF-C Activator 1 Homolog; RF-C/Activator 1 Homolog; RAD1 (S. Pombe) Homolog; Rad17-Like Protein; RAD17 Homolog; RAD1 Homolog; HRAD17; HRad17
Background

Gene Name: RAD17

NCBI Gene Entry: 5884

UniProt Entry: O75943
Application Information

Molecular Weight: Predicted, 77 kDa, observed, 85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB795

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-Rad17 (S656)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-Rad17 (S656) expression in HAP-1 cells using anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000). Green, isotype control; red, Phospho-Rad17 (S656).
  • Immunocytochemical staining of HAP-1 cells with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Phospho-Rad17 (S656) antibody (Cat#62429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-phospho-Rad17 (S656) antibody (Cat#62429). Phospho-Rad17 (S656) expression in wild-type (WT) and RAD17 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of Rad17 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-Rad17 (S656) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    RAD17
  • 货号：
    62429
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-Rad17 (S656) expression in HAP-1 cells using anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000). Green, isotype control; red, Phospho-Rad17 (S656).
Immunocytochemical staining of HAP-1 cells with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-Rad17 (S656) antibody (Cat#62429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-Rad17 (S656) antibody (Cat#62429). Phospho-Rad17 (S656) expression in wild-type (WT) and RAD17 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Rad17 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-Rad17 (S656) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-Rad17 (S656) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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