Western blotting analysis using anti-HNRNPH1 antibody (Cat#65063). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPH1 antibody (Cat#65063, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HNRNPH1 antibody (Cat#65063). HNRNPH1 expression in wild-type (WT) and HNRNPH1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HNRNPH1 antibody (Cat#65063, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of HNRNPH1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HNRNPH1 antibody (Cat#65063, 1:2,000) and analyzed using BD flow cytometer.
