Flow cytometric analysis of RALBP1 expression in HeLa cells using RALBP1 antibody (Cat#62445, 1:2,000). Green, isotype control; red, RALBP1.
Immunocytochemical staining of Hela cells with anti-RALBP1 antibody (Cat#62445, 1:1,000). Nuclei were stained blue with DAPI; RALBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-RALBP1 antibody (Cat#62445). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RALBP1 antibody (Cat#62445, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RALBP1 antibody (Cat#62445). RALBP1 expression in wild type (WT) and RALBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RALBP1 antibody (Cat#62445, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-RALBP1 antibody (Cat#62445, 1:1,000), Top panel: wild-type (WT); Bottom panal: RALBP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
