Flow cytometric analysis of PTPN11 expression in HepG2 cells using anti-PTPN11 antibody (Cat#63323, 1:2,000). Green, isotype control; red,PTPN11.
Immunocytochemical staining of HepG2 cells with anti-PTPN11 protein antibody(Cat #63323, 1:1,000) . Nuclei were stained blue with DAPI; PTPN11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PTPN11 antibody (Cat#63323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTPN11 antibody (Cat#63323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). PTPN11, protein tyrosine phosphatase non-receptor type 11.
Western blotting analysis using anti-PTPN11 antibody (Cat#63323). PTPN11 expression in wild type (WT) and PTPN11 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PTPN11 antibody (Cat#63323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
