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KD-Validated Anti-PTBP1 Rabbit Monoclonal Antibody #62491

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Flow cytometric analysis of PTBP1 expression in HAP-1 cells using anti-PTBP1 antibody (Cat#62491, 1:2,000). Green, isotype control; red, PTBP1.
Immunocytochemical staining of HAP1 cells with anti-PTBP1 antibody (Cat#62491, 1:1,000) . Nuclei were stained blue with DAPI; PTBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PTBP1 antibody (Cat#62491). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTBP1 antibody (Cat#62491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PTBP1 antibody (Cat#62491). PTBP1 expression in wild-type (WT) and PTBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PTBP1 antibody (Cat#62491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of PTBP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PTBP1 antibody (Cat#62491, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-PTBP1 antibody (Cat#62491, 1:1,000), Top panel: wild-type (WT); Bottom panal: PTBP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;PTBP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名：
    PTBP1
  • 货号：
    62491
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-PTBP1 Rabbit Monoclonal Antibody
Aliases Polypyrimidine Tract Binding Protein 1; Heterogeneous Nuclear Ribonucleoprotein I; HNRNP-I; HNRPI; PTB-1; PTB2; PTB3; PTB4; PPTB; PTB; Polypyrimidine Tract Binding Protein (Heterogeneous Nuclear Ribonucleoprotein I); Polypyrimidine Tract-Binding Protein 1; 57 KDa RNA-Binding Protein PPTB-1; HnRNP I; Heterogeneous Nuclear Ribonucleoprotein Polypeptide I; RNA-Binding Protein; HNRNPI; PTB-T
Background

Gene Name: PTBP1

NCBI Gene Entry: 5725

UniProt Entry: P26599
Application Information

Molecular Weight: Predicted, 60 kDa, observed, 57 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1095

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human PTBP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PTBP1 expression in HAP-1 cells using anti-PTBP1 antibody (Cat#62491, 1:2,000). Green, isotype control; red, PTBP1.
  • Immunocytochemical staining of HAP1 cells with anti-PTBP1 antibody (Cat#62491, 1:1,000) . Nuclei were stained blue with DAPI; PTBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-PTBP1 antibody (Cat#62491). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTBP1 antibody (Cat#62491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-PTBP1 antibody (Cat#62491). PTBP1 expression in wild-type (WT) and PTBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PTBP1 antibody (Cat#62491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of PTBP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PTBP1 antibody (Cat#62491, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-PTBP1 antibody (Cat#62491, 1:1,000), Top panel: wild-type (WT); Bottom panal: PTBP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;PTBP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
  • 基因名：
    PTBP1
  • 货号：
    62491
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of PTBP1 expression in HAP-1 cells using anti-PTBP1 antibody (Cat#62491, 1:2,000). Green, isotype control; red, PTBP1.
Immunocytochemical staining of HAP1 cells with anti-PTBP1 antibody (Cat#62491, 1:1,000) . Nuclei were stained blue with DAPI; PTBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PTBP1 antibody (Cat#62491). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTBP1 antibody (Cat#62491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PTBP1 antibody (Cat#62491). PTBP1 expression in wild-type (WT) and PTBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PTBP1 antibody (Cat#62491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of PTBP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PTBP1 antibody (Cat#62491, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-PTBP1 antibody (Cat#62491, 1:1,000), Top panel: wild-type (WT); Bottom panal: PTBP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;PTBP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-PTBP1 antibody (Cat#62491, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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