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KD-Validated Anti-SFPQ Rabbit Monoclonal Antibody #62518

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Flow cytometric analysis of SFPQ expression in HepG2 cells using anti-SFPQ antibody (Cat#62518, 1:2,000). Green, isotype control; red, SFPQ.
Immunocytochemical staining of HepG2 cells with anti-SFPQ antibody (Cat#62518, 1:1,000). Nuclei were stained blue with DAPI; SFPQ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-SFPQ antibody (Cat#62518). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SFPQ antibody (Cat#62518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SFPQ antibody (Cat#62518). SFPQ expression in wild type (WT) and SFPQ shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SFPQ antibody (Cat#62518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    SFPQ
  • 货号：
    62518
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-SFPQ Rabbit Monoclonal Antibody
Aliases Splicing Factor Proline And Glutamine Rich; PSF; PPP1R140; Polypyrimidine Tract Binding Protein Associated; Protein Phosphatase 1, Regulatory Subunit 140; DNA-Binding P52/P100 Complex, 100 KDa Subunit; Splicing Factor, Proline- And Glutamine-Rich; Splicing Factor Proline/Glutamine-Rich; 100 KDa DNA-Pairing Protein; Splicing Factor Proline/Glutamine Rich (Polypyrimidine Tract-Binding Protein-Associated); Splicing Factor Proline/Glutamine Rich (Polypyrimidine Tract Binding Protein Associated); Polypyrimidine Tract-Binding Protein-Associated Splicing Factor; Polypyrimidine Tract-Binding Protein-Associated-Splicing Factor; Epididymis Secretory Sperm Binding Protein; PTB-Associated Splicing Factor; PTB-Associated-Splicing Factor; HPOMp100; POMP100
Background

Gene Name: SFPQ

NCBI Gene Entry: 6421

UniProt Entry: P23246
Application Information

Molecular Weight: Predicted, 76 kDa, observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1220

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human SFPQ
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of SFPQ expression in HepG2 cells using anti-SFPQ antibody (Cat#62518, 1:2,000). Green, isotype control; red, SFPQ.
  • Immunocytochemical staining of HepG2 cells with anti-SFPQ antibody (Cat#62518, 1:1,000). Nuclei were stained blue with DAPI; SFPQ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-SFPQ antibody (Cat#62518). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SFPQ antibody (Cat#62518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-SFPQ antibody (Cat#62518). SFPQ expression in wild type (WT) and SFPQ shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SFPQ antibody (Cat#62518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    SFPQ
  • 货号：
    62518
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of SFPQ expression in HepG2 cells using anti-SFPQ antibody (Cat#62518, 1:2,000). Green, isotype control; red, SFPQ.
Immunocytochemical staining of HepG2 cells with anti-SFPQ antibody (Cat#62518, 1:1,000). Nuclei were stained blue with DAPI; SFPQ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-SFPQ antibody (Cat#62518). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SFPQ antibody (Cat#62518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SFPQ antibody (Cat#62518). SFPQ expression in wild type (WT) and SFPQ shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SFPQ antibody (Cat#62518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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