KD-Validated Anti-Phospho-EGFR (Y1068) Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-Phospho-EGFR (Y1068) Rabbit Monoclonal Antibody #61206

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Western blotting analysis using anti-phospho-EGFR (Y1068) antibody (Cat#61206). Phospho-EGFR (Y1068) expression in wild type (WT) and EGFR shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-EGFR (Y1068) antibody (Cat#61206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-phospho-EGFR (Y1068) antibody (Cat#61206). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-EGFR (Y1068) antibody (Cat#61206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Validation of EGFR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with anti-Phospho-EGFR (Y1092) antibody (Cat#61206, 1:2,000) and analyzed using BD flow cytometer.

基因名：

EGFR
货号：

61206
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

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Citation(0)

Information

Product Name	KD-Validated Anti-Phospho-EGFR (Y1068) Rabbit Monoclonal Antibody
Aliases	EGFR; Epidermal Growth Factor Receptor; ERBB1; ERRP; ERBB; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Epidermal Growth Factor Receptor Tyrosine Kinase Domain; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EGFR VIII; EC 2.7.10; NISBD2; PIG61; MENA
Background	Gene Name: EGFR NCBI Gene Entry: 1956 UniProt Entry: P00533
Application Information	Molecular Weight: Predicted, 134 kDa; observed, 175 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB16190 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Phospho-EGFR (Y1068)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-phospho-EGFR (Y1068) antibody (Cat#61206). Phospho-EGFR (Y1068) expression in wild type (WT) and EGFR shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-EGFR (Y1068) antibody (Cat#61206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-EGFR (Y1068) antibody (Cat#61206). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-EGFR (Y1068) antibody (Cat#61206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of EGFR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with anti-Phospho-EGFR (Y1092) antibody (Cat#61206, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

EGFR
货号：

61206
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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