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KD-Validated Anti-Synaptosome Associated Protein 29 Rabbit Monoclonal Antibody #62640

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Flow cytometric analysis of Synaptosome associated protein 29 expression in HepG2 cells using anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:2,000). Green, isotype control; red, Synaptosome associated protein 29.
Immunocytochemical staining of HepG2 cells with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:1,000). Nuclei were stained blue with DAPI; Synaptosome associated protein 29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Synaptosome associated protein 29 antibody (Cat#62640). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Synaptosome associated protein 29 antibody (Cat#62640). Synaptosome associated protein 29 expression in wild type (WT) and Synaptosome associated protein 29 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    SNAP29
  • 货号：
    62640
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Synaptosome Associated Protein 29 Rabbit Monoclonal Antibody
Aliases SNAP29; Synaptosome Associated Protein 29; SNAP-29; Soluble 29 KDa NSF Attachment Protein; CEDNIK; Cerebral Dysgenesis, Neuropathy, Ichthyosis And Keratoderma Syndrome; Vesicle-Membrane Fusion Protein SNAP-29; Synaptosomal-Associated Protein, 29kDa; Synaptosomal-Associated Protein 29; Synaptosomal-Associated Protein, 29kD; Synaptosome Associated Protein 29kDa
Background

Gene Name: SNAP29

NCBI Gene Entry: 9342

UniProt Entry: O95721
Application Information

Molecular Weight: Predicted, 29 kDa, observed, 27 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1855

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human SNAP29
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Synaptosome associated protein 29 expression in HepG2 cells using anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:2,000). Green, isotype control; red, Synaptosome associated protein 29.
  • Immunocytochemical staining of HepG2 cells with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:1,000). Nuclei were stained blue with DAPI; Synaptosome associated protein 29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Synaptosome associated protein 29 antibody (Cat#62640). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Synaptosome associated protein 29 antibody (Cat#62640). Synaptosome associated protein 29 expression in wild type (WT) and Synaptosome associated protein 29 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    SNAP29
  • 货号：
    62640
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Synaptosome associated protein 29 expression in HepG2 cells using anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:2,000). Green, isotype control; red, Synaptosome associated protein 29.
Immunocytochemical staining of HepG2 cells with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:1,000). Nuclei were stained blue with DAPI; Synaptosome associated protein 29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Synaptosome associated protein 29 antibody (Cat#62640). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Synaptosome associated protein 29 antibody (Cat#62640). Synaptosome associated protein 29 expression in wild type (WT) and Synaptosome associated protein 29 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Synaptosome associated protein 29 antibody (Cat#62640, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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