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Anti-Adenosine Deaminase RNA Specific Rabbit Monoclonal Antibody #3028

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Flow cytometric analysis of Adenosine deaminase RNA specific expression in HepG2 cells using anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:2,000). Green, isotype control; red, Adenosine deaminase RNA specific.
Immunocytochemical staining of HepG2 cells with anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:1,000) . Nuclei were stained blue with DAPI; Adenosine deaminase RNA specific was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Adenosine deaminase RNA specific antibody (Cat#3028). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    ADAR
  • 货号:
    3028
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Adenosine Deaminase RNA Specific Rabbit Monoclonal Antibody
Aliases ADAR; Adenosine Deaminase RNA Specific; ADAR1; DRADA; Double-Stranded RNA-Specific Adenosine Deaminase; G1P1; IFI4; 136 KDa Double-Stranded RNA-Binding Protein; Interferon-Inducible Protein 4; Interferon-Induced Protein 4; K88DSRBP; DSRAD; IFI-4; P136; Adenosine Deaminase Acting On RNA 1-A; DsRNA Adeonosine Deaminase; DsRNA Adenosine Deaminase; EC 3.5.4.37; EC 3.5.4; AGS6; DSH
Background

Gene Name: ADAR

NCBI Gene Entry: 103

UniProt Entry: P55265

Application Information

Molecular Weight: Predicted, 136 kDa; observed, 150 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3705

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human ADAR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Adenosine deaminase RNA specific expression in HepG2 cells using anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:2,000). Green, isotype control; red, Adenosine deaminase RNA specific.
  • Immunocytochemical staining of HepG2 cells with anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:1,000) . Nuclei were stained blue with DAPI; Adenosine deaminase RNA specific was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Adenosine deaminase RNA specific antibody (Cat#3028). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Adenosine deaminase RNA specific expression in HepG2 cells using anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:2,000). Green, isotype control; red, Adenosine deaminase RNA specific.
Immunocytochemical staining of HepG2 cells with anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:1,000) . Nuclei were stained blue with DAPI; Adenosine deaminase RNA specific was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Adenosine deaminase RNA specific antibody (Cat#3028). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adenosine deaminase RNA specific antibody (Cat#3028, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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