Flow cytometric analysis of TMF expression in HepG2 cells using anti-TMF antibody (Cat#62805, 1:2,000). Green, isotype control; red, TMF.
Immunocytochemical staining of HepG2 cells with anti-TMF antibody (Cat#62805, 1:1,000). Nuclei were stained blue with DAPI; TMF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-TMF antibody (Cat#62805). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TMF antibody (Cat#62805, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TMF antibody (Cat#62805). TMF expression in wild-type (WT) and TMF1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TMF antibody (Cat#62805, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). TMF1, TATA element modulatory factor 1.
Validation of TMF knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-TMF antibody (Cat#62805, 1:2,000) and analyzed using BD flow cytometer.
