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KO-Validated Anti-ATP1A1 Rabbit Monoclonal Antibody #71282

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Western blotting analysis using anti-ATP1A1 antibody (Cat#71282). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ATP1A1 antibody (Cat#71282). ATP1A1 expression in wild-type (WT) and ATP1A1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    ATP1A1
  • 货号:
    71282
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-ATP1A1 Rabbit Monoclonal Antibody
Aliases ATP1A1; ATPase Na+/K+ Transporting Subunit Alpha 1; Sodium/Potassium-Transporting ATPase Subunit Alpha-1; Sodium Pump Subunit Alpha-1; Sodium-Potassium ATPase Catalytic Subunit Alpha-1; ATPase, Na+/K+ Transporting, Alpha 1 Polypeptide; Na(+)/K(+) ATPase Alpha-1 Subunit; Na,K-ATPase Catalytic Subunit Alpha-A Protein; Sodium-Potassium-ATPase, Alpha 1 Polypeptide; Na, K-ATPase, Alpha-A Catalytic Polypeptide; Na,K-ATPase Alpha-1 Subunit; Na+/K+ ATPase 1; EC 7.2.2.13; EC 3.6.3.9; HOMGSMR2; EC 3.6.3; CMT2DD
Background

Gene Name: ATP1A1

NCBI Gene Entry: 476

UniProt Entry: P05023

Application Information

Molecular Weight: Predicted, 113 kDa; observed, 85,110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6185

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Sodium Potassium ATPase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-ATP1A1 antibody (Cat#71282). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-ATP1A1 antibody (Cat#71282). ATP1A1 expression in wild-type (WT) and ATP1A1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Western blotting analysis using anti-ATP1A1 antibody (Cat#71282). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ATP1A1 antibody (Cat#71282). ATP1A1 expression in wild-type (WT) and ATP1A1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP1A1 antibody (Cat#71282, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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