Anti-Cyclin Dependent Kinase Inhibitor 1A Rabbit Monoclonal Antibody - 产品中心

Anti-Cyclin Dependent Kinase Inhibitor 1A Rabbit Monoclonal Antibody #3071

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Flow cytometric analysis of p21 expression in HT-1080 cells using anti-p21 antibody (Cat#3071, 1:2,000). Green, isotype control; red, p21.

Immunocytochemical staining of HT-1080 cells with anti-p21 antibody (Cat#3071, 1:1,000) . Nuclei were stained blue with DAPI; p21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-p21 antibody (Cat#3071). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p21 antibody (Cat#3071, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CDKN1A
货号：

3071
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Cyclin Dependent Kinase Inhibitor 1A Rabbit Monoclonal Antibody
Aliases	CDKN1A; Cyclin Dependent Kinase Inhibitor 1A; CAP20; CIP1; WAF1; SDI1; P21; P21CIP1; CDKN1; Cyclin-Dependent Kinase Inhibitor 1A (P21, Cip1); Cyclin-Dependent Kinase Inhibitor 1; Cdk-Interacting Protein 1; P21Cip1/Waf1; MDA-6; Melanoma Differentiation Associated Protein 6; Melanoma Differentiation-Associated Protein 6; Wild-Type P53-Activated Fragment 1; CDK-Interaction Protein 1; CDK-Interacting Protein 1; DNA Synthesis Inhibitor; MDA6; PIC1
Background	Gene Name: CDKN1A NCBI Gene Entry: 1026 UniProt Entry: P38936
Application Information	Molecular Weight: Predicted, 18 kDa; observed, 18 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3920 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human p21
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of p21 expression in HT-1080 cells using anti-p21 antibody (Cat#3071, 1:2,000). Green, isotype control; red, p21.
Immunocytochemical staining of HT-1080 cells with anti-p21 antibody (Cat#3071, 1:1,000) . Nuclei were stained blue with DAPI; p21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-p21 antibody (Cat#3071). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p21 antibody (Cat#3071, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CDKN1A
货号：

3071
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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