Flow cytometric analysis of Caspase 9 expression in HepG2 cells using anti-Caspase 9 antibody (Cat#63156, 1:2,000). Green, isotype control; red,Caspase 9.
Immunocytochemical staining of HepG2 cells with anti-Caspase 9 antibody(Cat#63156, 1:1,000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-caspase 9 antibody (Cat#63156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 9 antibody (Cat#63156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-caspase 9 antibody (Cat#63156). Caspase 9 expression in wild type (WT) and caspase 9 (CASP9) shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-caspase 9 antibody (Cat#63156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).