Flow cytometric analysis of catalase expression in H9c2 cells using anti-catalase antibody (Cat#63157, 1:2,000). Green, isotype control; red, catalase.
Immunocytochemical staining of H9C2 cells with anti-Catalase antibody (Cat#63157, 1:1,000) . Nuclei were stained blue with DAPI; Catalase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-catalase antibody (Cat#63157). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-catalase antibody (Cat#63157, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-catalase antibody (Cat#63157). Catalase expression in wild type (WT) and catalase (CAT) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-catalase antibody (Cat#63157, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Catalase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Catalase antibody (Cat#63157, 1:2,000) and analyzed using BD flow cytometer.
