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Anti-EOGT Rabbit Monoclonal Antibody #4419

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Flow cytometric analysis of EOGT expression in HT-1080 cells using anti-EOGT antibody (Cat#4419, 1:2,000). Green, isotype control; red, EOGT.

Immunocytochemical staining of HT-1080 cells with anti-EOGT antibody (Cat#4419, 1:1000). Nuclei were stained blue with DAPI; EOGT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-EOGT antibody (Cat#4419). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EOGT antibody (Cat#4419, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

EOGT
货号：

4419
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-EOGT Rabbit Monoclonal Antibody
Aliases	EOGT; EGF Domain Specific O-Linked N-Acetylglucosamine Transferase; AER61; EGF Domain-Specific O-Linked N-Acetylglucosamine (GlcNAc) Transferase; EGF Domain-Specific O-Linked N-Acetylglucosamine Transferase; Extracellular O-Linked N-Acetylglucosamine Transferase; AER61 Glycosyltransferase; FLJ33770; C3orf64; EOGT1; Chromosome 3 Open Reading Frame 64; EGF-O-GlcNAc Transferase; EC 2.4.1.255; C3ORF64; AOS4
Background	Gene Name: EOGT NCBI Gene Entry: 285203 UniProt Entry: Q5NDL2
Application Information	Molecular Weight: Predicted, 62 kDa; observed, 55 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11125 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human AER61
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of EOGT expression in HT-1080 cells using anti-EOGT antibody (Cat#4419, 1:2,000). Green, isotype control; red, EOGT.
Immunocytochemical staining of HT-1080 cells with anti-EOGT antibody (Cat#4419, 1:1000). Nuclei were stained blue with DAPI; EOGT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-EOGT antibody (Cat#4419). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EOGT antibody (Cat#4419, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

EOGT
货号：

4419
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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