Flow cytometric analysis of EGFR expression in HepG2 cells using anti-EGFR antibody (Cat#63180, 1:2,000). Green, isotype control; red, EGFR.
Immunocytochemical staining of HepG2 cells with anti-EGFR antibody (Cat#63180, 1:1,000). Nuclei were stained blue with DAPI; EGFR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-EGFR antibody (Cat#63180). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EGFR antibody (Cat#63180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-EGFR antibody (Cat#63180). EGFR expression in wild type (WT) and EGFR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EGFR antibody (Cat#63180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells using anti-EGFR antibody (Cat#63180, 1:1,000), Top panel: wild-type (WT); Bottom panal: EGFR shRNA knockdown (KD). Nuclei were stained blue with DAPI; EGFR was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
