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KD-Validated Anti-G Protein-Coupled Receptor Kinase 6 Rabbit Monoclonal Antibody #63283

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Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HepG2 cells using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:2,000). Green, isotype control; red, G protein-coupled receptor kinase 6.
Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). G protein-coupled receptor kinase 6 expression in wild type (WT) and G protein-coupled receptor kinase 6 (GRK6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of G protein-coupled receptor kinase 6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名:
    GRK6
  • 货号:
    63283
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-G Protein-Coupled Receptor Kinase 6 Rabbit Monoclonal Antibody
Aliases GRK6; G Protein-Coupled Receptor Kinase 6; GPRK6; EC 2.7.11.16; G Protein-Coupled Receptor Kinase GRK6; EC 2.7.11
Background

Gene Name: GRK6

NCBI Gene Entry: 2870

UniProt Entry: P43250

Application Information

Molecular Weight: Predicted, 66 kDa, observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4870

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human GRK6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HepG2 cells using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:2,000). Green, isotype control; red, G protein-coupled receptor kinase 6.
  • Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). G protein-coupled receptor kinase 6 expression in wild type (WT) and G protein-coupled receptor kinase 6 (GRK6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of G protein-coupled receptor kinase 6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HepG2 cells using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:2,000). Green, isotype control; red, G protein-coupled receptor kinase 6.
Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). G protein-coupled receptor kinase 6 expression in wild type (WT) and G protein-coupled receptor kinase 6 (GRK6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of G protein-coupled receptor kinase 6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:2,000) and analyzed using BD flow cytometer.
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