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KD-Validated Anti-Mitotic Arrest Deficient 2 Like 2 Rabbit Monoclonal Antibody #63299

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Flow cytometric analysis of MAD2L2 expression in HAP-1 cells using anti-MAD2L2 antibody (Cat#63299, 1:2,000). Green, isotype control; red,MAD2L2 .
Immunocytochemical staining of HAP1 cells with anti-MAD2L2 antibody (Cat#63299, 1:1,000) . Nuclei were stained blue with DAPI;MAD2L2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MAD2L2 antibody (Cat#63299). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAD2L2 antibody (Cat#63299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). MAD2L2, mitotic arrest deficient 2 like 2.
Western blotting analysis using anti-MAD2L2 antibody (Cat#63299). MAD2L2 expression in wild-type (WT) and MAD2L2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MAD2L2 antibody (Cat#63299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    MAD2L2
  • 货号：
    63299
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Mitotic Arrest Deficient 2 Like 2 Rabbit Monoclonal Antibody
Aliases MAD2L2; Mitotic Arrest Deficient 2 Like 2; MAD2B; REV7; POLZ2; FANCV; MAD2 (Mitotic Arrest Deficient, Yeast, Homolog)-Like 2; Polymerase (DNA-Directed), Zeta 2, Accessory Subunit; Mitotic Spindle Assembly Checkpoint Protein MAD2B; Mitotic Arrest Deficient 2-Like Protein 2; Mitotic Arrest Deficient Homolog-Like 2; MAD2-Like Protein 2; REV7 Homolog; HREV7; MAD2 Mitotic Arrest Deficient-Like 2
Background

Gene Name: MAD2L2

NCBI Gene Entry: 10459

UniProt Entry: Q9UI95
Application Information

Molecular Weight: Predicted, 24 kDa, observed, 24 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4950

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Mad2L2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MAD2L2 expression in HAP-1 cells using anti-MAD2L2 antibody (Cat#63299, 1:2,000). Green, isotype control; red,MAD2L2 .
  • Immunocytochemical staining of HAP1 cells with anti-MAD2L2 antibody (Cat#63299, 1:1,000) . Nuclei were stained blue with DAPI;MAD2L2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-MAD2L2 antibody (Cat#63299). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAD2L2 antibody (Cat#63299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). MAD2L2, mitotic arrest deficient 2 like 2.
  • Western blotting analysis using anti-MAD2L2 antibody (Cat#63299). MAD2L2 expression in wild-type (WT) and MAD2L2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MAD2L2 antibody (Cat#63299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    MAD2L2
  • 货号：
    63299
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of MAD2L2 expression in HAP-1 cells using anti-MAD2L2 antibody (Cat#63299, 1:2,000). Green, isotype control; red,MAD2L2 .
Immunocytochemical staining of HAP1 cells with anti-MAD2L2 antibody (Cat#63299, 1:1,000) . Nuclei were stained blue with DAPI;MAD2L2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MAD2L2 antibody (Cat#63299). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAD2L2 antibody (Cat#63299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). MAD2L2, mitotic arrest deficient 2 like 2.
Western blotting analysis using anti-MAD2L2 antibody (Cat#63299). MAD2L2 expression in wild-type (WT) and MAD2L2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MAD2L2 antibody (Cat#63299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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