Flow cytometric analysis of Mitofusin 1 expression in C2C12 cells using anti-Mitofusin 1 antibody (Cat#63303, 1:2,000). Green, isotype control; red, Mitofusin 1.
Immunocytochemical staining of C2C12 cells with anti-Mitofusin 1 antibody (Cat#63303, 1:1,000) . Nuclei were stained blue with DAPI; Mitofusin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-mitofusin 1 antibody (Cat#63303). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-mitofusin 1 antibody (Cat#63303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-mitofusin 1 antibody (Cat#63303). Mitofusin 1 expression in wild-type (WT) and mitofusin 1 (MFN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-mitofusin 1 antibody (Cat#63303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Mitofusin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Mitofusin 1 antibody (Cat#63303, 1:2,000) and analyzed using BD flow cytometer.
