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KD-Validated Anti-Phospho-HSF1 (Ser326) Rabbit Monoclonal Antibody #63380

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Flow cytometric analysis of Phospho-HSF1 (Ser326) expression in HepG2 cells using anti-Phospho-HSF1 (Ser326) antibody (Cat#63380, 1:2,000). Green, isotype control; red,Phospho-HSF1 (Ser326).
Immunocytochemical staining of HepG2 cells with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:1,000). Nuclei were stained blue with DAPI; Phospho-HSF1 (Ser326) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-HSF1 (Ser326) antibody (Cat#63380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-HSF1 (Ser326) antibody (Cat#63380). Phospho-HSF1 (Ser326) expression in wild type (WT) and HSF1 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). HSF1, heat shock transcription factor 1.
  • 基因名：
    HSF1
  • 货号：
    63380
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-HSF1 (Ser326) Rabbit Monoclonal Antibody
Aliases HSF1; Heat Shock Transcription Factor 1; HSTF1; Heat Shock Factor Protein 1; HSTF; HSF
Background

Gene Name: HSF1

NCBI Gene Entry: 3297

UniProt Entry: Q00613
Application Information

Molecular Weight: Predicted, 57 kDa, observed, 82 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5400

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-HSF1 (Ser326)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-HSF1 (Ser326) expression in HepG2 cells using anti-Phospho-HSF1 (Ser326) antibody (Cat#63380, 1:2,000). Green, isotype control; red,Phospho-HSF1 (Ser326).
  • Immunocytochemical staining of HepG2 cells with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:1,000). Nuclei were stained blue with DAPI; Phospho-HSF1 (Ser326) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-phospho-HSF1 (Ser326) antibody (Cat#63380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-phospho-HSF1 (Ser326) antibody (Cat#63380). Phospho-HSF1 (Ser326) expression in wild type (WT) and HSF1 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). HSF1, heat shock transcription factor 1.
Citation(0)
  • 基因名：
    HSF1
  • 货号：
    63380
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-HSF1 (Ser326) expression in HepG2 cells using anti-Phospho-HSF1 (Ser326) antibody (Cat#63380, 1:2,000). Green, isotype control; red,Phospho-HSF1 (Ser326).
Immunocytochemical staining of HepG2 cells with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:1,000). Nuclei were stained blue with DAPI; Phospho-HSF1 (Ser326) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-HSF1 (Ser326) antibody (Cat#63380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-HSF1 (Ser326) antibody (Cat#63380). Phospho-HSF1 (Ser326) expression in wild type (WT) and HSF1 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-HSF1 (Ser326) antibody (Cat#63380, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). HSF1, heat shock transcription factor 1.
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