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Anti-eIF3A Rabbit Monoclonal Antibody #9323

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Flow cytometric analysis of eIF3A expression in HT-1080 cells using eIF3A antibody (Cat#9323, 1:2,000). Green, isotype control; red, eIF3A.
Immunocytochemical staining of HT-1080 cells with eIF3A antibody (Cat#9323, 1:1,000). Nuclei were stained blue with DAPI; eIF3A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-eIF3A antibody (Cat#9323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-eIF3A antibody (Cat#9323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    EIF3A
  • 货号：
    9323
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-eIF3A Rabbit Monoclonal Antibody
Aliases Eukaryotic Translation Initiation Factor 3 Subunit A; EIF3-Theta; EIF3-P170; KIAA0139; EIF3S10; TIF32; Eukaryotic Translation Initiation Factor 3, Subunit 10 Theta, 150/170kDa; Eukaryotic Translation Initiation Factor 3 Subunit 10; EIF-3-Theta; EIF3 P167; EIF3 P180; EIF3 P185; EIF3; Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 150/170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10, 170kD; Eukaryotic Translation Initiation Factor 3, Subunit A; Cytoplasmic Protein P167; Centrosomin Homolog; EIF3, P180 Subunit; EIF3a; P167; P180; P185
Background

Gene Name: EIF3A

NCBI Gene Entry: 8661

UniProt Entry: Q14152
Application Information

Molecular Weight: Predicted, 167 kDa; observed, 160 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB830

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human eIF3A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of eIF3A expression in HT-1080 cells using eIF3A antibody (Cat#9323, 1:2,000). Green, isotype control; red, eIF3A.
  • Immunocytochemical staining of HT-1080 cells with eIF3A antibody (Cat#9323, 1:1,000). Nuclei were stained blue with DAPI; eIF3A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-eIF3A antibody (Cat#9323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-eIF3A antibody (Cat#9323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    EIF3A
  • 货号：
    9323
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of eIF3A expression in HT-1080 cells using eIF3A antibody (Cat#9323, 1:2,000). Green, isotype control; red, eIF3A.
Immunocytochemical staining of HT-1080 cells with eIF3A antibody (Cat#9323, 1:1,000). Nuclei were stained blue with DAPI; eIF3A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-eIF3A antibody (Cat#9323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-eIF3A antibody (Cat#9323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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