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KD-Validated Anti-NK2 Homeobox 1 Rabbit Monoclonal Antibody #63388

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Flow cytometric analysis of NK2 homeobox 1 expression in HAP-1 cells using anti-NK2 homeobox 1 antibody (Cat#63388, 1:2,000). Green, isotype control; red, NK2 homeobox 1.
Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). NK2 homeobox 1 expression in wild type (WT) and NK2 homeobox 1 (NKX2-1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    NKX2-1
  • 货号:
    63388
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-NK2 Homeobox 1 Rabbit Monoclonal Antibody
Aliases NKX2-1; NK2 Homeobox 1; TTF-1; TTF1; Thyroid Transcription Factor 1; NKX2A; TITF1; Thyroid-Specific Enhancer-Binding Protein; Homeobox Protein NK-2 Homolog A; Homeobox Protein Nkx-2.1; Thyroid Nuclear Factor 1; T/EBP; BCH; NK-2 Homolog A; Benign Chorea; NKX2.1; NMTC1; NK-2; TEBP; BHC
Background

Gene Name: NKX2-1

NCBI Gene Entry: 7080

UniProt Entry: P43699

Application Information

Molecular Weight: Predicted, 39 kDa, observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5440

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human TTF1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NK2 homeobox 1 expression in HAP-1 cells using anti-NK2 homeobox 1 antibody (Cat#63388, 1:2,000). Green, isotype control; red, NK2 homeobox 1.
  • Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). NK2 homeobox 1 expression in wild type (WT) and NK2 homeobox 1 (NKX2-1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of NK2 homeobox 1 expression in HAP-1 cells using anti-NK2 homeobox 1 antibody (Cat#63388, 1:2,000). Green, isotype control; red, NK2 homeobox 1.
Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). NK2 homeobox 1 expression in wild type (WT) and NK2 homeobox 1 (NKX2-1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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