Flow cytometric analysis of NK2 homeobox 1 expression in HAP-1 cells using anti-NK2 homeobox 1 antibody (Cat#63388, 1:2,000). Green, isotype control; red, NK2 homeobox 1.
Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). NK2 homeobox 1 expression in wild type (WT) and NK2 homeobox 1 (NKX2-1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).