Immunocytochemical analysis of BCKDHA staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of BCKDHA expression in SGC7901 (A), mouse liver (B), mouse spleen (C), rat liver (D), rat spleen (E) whole cell lysates.
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
Recombinant protein of human BCKDHA
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunocytochemistry (IC): 1:50-1:200
Note
This product is for research use only.
Picture
Immunocytochemical analysis of BCKDHA staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of BCKDHA expression in SGC7901 (A), mouse liver (B), mouse spleen (C), rat liver (D), rat spleen (E) whole cell lysates.
Immunocytochemical analysis of BCKDHA staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of BCKDHA expression in SGC7901 (A), mouse liver (B), mouse spleen (C), rat liver (D), rat spleen (E) whole cell lysates.
