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KD-Validated Anti-Caspase 3 Rabbit Monoclonal Antibody #63400

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Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-caspase 3 antibody (Cat#63400, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using anti-caspase 3 antibody (Cat#63400). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 3 antibody (Cat#63400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-caspase 3 antibody (Cat#63400). Caspase 3 expression in wild-type (WT) and caspase 3 (CASP3) shRNA knockdown (KD) HAP-1 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-caspase 3 antibody (Cat#63400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CASP3
货号：

63400
应用：

WB, IHC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-Caspase 3 Rabbit Monoclonal Antibody
Aliases	CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22
Background	Gene Name: CASP3 NCBI Gene Entry: 836 UniProt Entry: P42574
Application Information	Molecular Weight: Predicted, 32 kDa, observed, 32 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6150 Species Reactivity: Human Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen	A synthesized peptide derived from human Caspase-3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunohistochemistry (IHC): 1:50-1:100
Note	This product is for research use only.

Picture

Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-caspase 3 antibody (Cat#63400, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-caspase 3 antibody (Cat#63400). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 3 antibody (Cat#63400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-caspase 3 antibody (Cat#63400). Caspase 3 expression in wild-type (WT) and caspase 3 (CASP3) shRNA knockdown (KD) HAP-1 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-caspase 3 antibody (Cat#63400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CASP3
货号：

63400
应用：

WB, IHC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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