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Anti-PI3K p110 α Rabbit Monoclonal Antibody #6343

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Flow cytometric analysis of PI3 kinase p110 alpha expression in H9c2 cells using PI3 kinase p110 alpha antibody (Cat#6343, 1:2,000). Green, isotype control; red, PI3 kinase p110 alpha.
Immunocytochemical staining of HepG2 cells with PI3 kinase p110 alpha antibody (Cat#6343, 1:1,000). Nuclei were stained blue with DAPI; PI3 kinase p110 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PI3K p110 α antibody (Cat#6343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3K p110 α antibody (Cat#6343, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    PIK3CA
  • 货号:
    6343
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-PI3K p110 α Rabbit Monoclonal Antibody
Aliases PI3K; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase Catalytic Subunit; Alpha Isoform; Phosphoinositide-3-Kinase, Catalytic, Alpha Polypeptide; Serine/Threonine Protein Kinase PIK3CA PtdIns-3-Kinase Subunit P110-Alpha; Phosphoinositide 3-Kinase Alpha; PI3K-Alpha; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Mutant Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase, Catalytic Subunit Alpha; Phosphatidylinositol 3-Kinase, Catalytic, Alpha Polypeptide; Phosphatidylinositol 3-Kinase, Catalytic, 110-KD, Alpha; Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide; PI3-Kinase P110 Subunit Alpha; PtdIns-3-Kinase Subunit Alpha; PI3-Kinase Subunit Alpha; EC 2.7.1.137; EC 2.7.1.153; EC 2.7.11.1; P110-Alpha; PI3Kalpha; P110alpha; EC 2.7.1 48; CLAPO; CLOVE; MCMTC; CCM4; CWS5; MCAP; MCM
Background

Gene Name: PIK3CA

NCBI Gene Entry: 5290

UniProt Entry: P42336

Application Information

Molecular Weight: Predicted, 124 kDa; observed, 110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB950

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PI3K p110 α
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PI3 kinase p110 alpha expression in H9c2 cells using PI3 kinase p110 alpha antibody (Cat#6343, 1:2,000). Green, isotype control; red, PI3 kinase p110 alpha.
  • Immunocytochemical staining of HepG2 cells with PI3 kinase p110 alpha antibody (Cat#6343, 1:1,000). Nuclei were stained blue with DAPI; PI3 kinase p110 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-PI3K p110 α antibody (Cat#6343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3K p110 α antibody (Cat#6343, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of PI3 kinase p110 alpha expression in H9c2 cells using PI3 kinase p110 alpha antibody (Cat#6343, 1:2,000). Green, isotype control; red, PI3 kinase p110 alpha.
Immunocytochemical staining of HepG2 cells with PI3 kinase p110 alpha antibody (Cat#6343, 1:1,000). Nuclei were stained blue with DAPI; PI3 kinase p110 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PI3K p110 α antibody (Cat#6343). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3K p110 α antibody (Cat#6343, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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