Immunocytochemical staining of HepG2 cells with anti-DSTN antibody (Cat#63439, 1:1,000) . Nuclei were stained blue with DAPI; DSTN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-DSTN antibody (Cat#63439). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DSTN antibody (Cat#63439, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-DSTN antibody (Cat#63439). DSTN expression in wild type (WT) and DSTN shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-DSTN antibody (Cat#63439, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of DSTN knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DSTN antibody (Cat#63439, 1:2,000) and analyzed using BD flow cytometer.
