Flow cytometric analysis of Protein phosphatase 5 catalytic subunit expression in HepG2 cells using anti-Protein phosphatase 5 catalytic subunit antibody (Cat#63496, 1:2,000). Green, isotype control; red, Protein phosphatase 5 catalytic subunit.
Western blotting analysis using anti-protein phosphatase 5 catalytic subunit antibody (Cat#63496). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-protein phosphatase 5 catalytic subunit antibody (Cat#63496, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-protein phosphatase 5 catalytic subunit antibody (Cat#63496). Protein Phosphatase 5 catalytic subunit expression in wild type (WT) and protein phosphatase 5 catalytic subunit (PPP5C) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-protein phosphatase 5 catalytic subunit antibody (Cat#63496, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
