Flow cytometric analysis of Rho GDP dissociation inhibitor alpha expression in HepG2 cells using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501, 1:2,000). Green, isotype control; red, Rho GDP dissociation inhibitor alpha.
Immunocytochemical staining of HepG2 cells with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501, 1:1,000) . Nuclei were stained blue with DAPI; Rho GDP dissociation inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501). Rho GDP dissociation inhibitor alpha expression in wild type (WT) and Rho GDP dissociation inhibitor alpha (ARHGDIA) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
