Flow cytometric analysis of ASCC2 expression in HepG2 cells using anti-ASCC2 antibody (Cat#63525, 1:2,000). Green, isotype control; red, ASCC2.
Immunocytochemical staining of HepG2 cells with anti-ASCC2 antibody (Cat#63525, 1:1,000) . Nuclei were stained blue with DAPI; ASCC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-ASCC2 antibody (Cat#63525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ASCC2 antibody (Cat#63525, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ASCC2 antibody (Cat#63525). ASCC2 expression in wild type (WT) and ASCC2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ASCC2 antibody (Cat#63525, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of ASCC2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ASCC2 antibody (Cat#63525, 1:2,000) and analyzed using BD flow cytometer.
