Flow cytometric analysis of Diacylglycerol kinase alpha expression in HT-1080 cells using anti-Diacylglycerol kinase alpha antibody (Cat#63540, 1:2,000). Green, isotype control; red, Diacylglycerol kinase alpha.
Western blotting analysis using anti-diacylglycerol kinase alpha antibody (Cat#63540). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-diacylglycerol kinase alpha antibody (Cat#63540, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-diacylglycerol kinase alpha antibody (Cat#63540). Diacylglycerol kinase alpha expression in wild type (WT) and diacylglycerol kinase alpha (DGKA) knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-diacylglycerol kinase alpha antibody (Cat#63540, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
