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KD-Validated Anti-HERPUD1 Mouse Monoclonal Antibody #65049

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Western blotting analysis using anti-HERPUD1 antibody (Cat#65049). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HERPUD1 antibody (Cat#65049, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HERPUD1 antibody (Cat#65049). HERPUD1 expression in wild-type (WT) and HERPUD1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HERPUD1 antibody (Cat#65049, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-HERPUD1 antibody (Cat#65049, 1:1,000), Top panel: wild-type (WT); Bottom panal: HERPUD1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HERPUD1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of HERPUD1 expression in C2C12 cells using anti-HERPUD1 antibody (Cat#65049, 1:1,000). Green, isotype control; red, HERPUD1.
  • 基因名：
    HERPUD1
  • 货号：
    65049
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-HERPUD1 Mouse Monoclonal Antibody
Aliases HERPUD1; Homocysteine Inducible ER Protein With Ubiquitin Like Domain 1; HERP; KIAA0025; Mif1; SUP; Homocysteine-Inducible, Endoplasmic Reticulum Stress-Inducible, Ubiquitin-Like Domain Member 1; Homocysteine-Responsive Endoplasmic Reticulum-Resident Ubiquitin-Like Domain Member 1 Protein; Methyl Methanesulfonate (MMF)-Inducible Fragment Protein 1; omocysteine-Inducible Endoplasmic Reticulum Stress-Inducible Ubiquitin-Like Domain Member 1 Protein; MMS-Inducible; MIF1
Background

Gene Name: HERPUD1

NCBI Gene Entry: 9709

UniProt Entry: Q15011
Application Information

Molecular Weight: Predicted, 44 kDa; observed, 54 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14035

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen Recombinant protein of human HERPUD1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-HERPUD1 antibody (Cat#65049). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HERPUD1 antibody (Cat#65049, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-HERPUD1 antibody (Cat#65049). HERPUD1 expression in wild-type (WT) and HERPUD1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HERPUD1 antibody (Cat#65049, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using anti-HERPUD1 antibody (Cat#65049, 1:1,000), Top panel: wild-type (WT); Bottom panal: HERPUD1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HERPUD1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • Flow cytometric analysis of HERPUD1 expression in C2C12 cells using anti-HERPUD1 antibody (Cat#65049, 1:1,000). Green, isotype control; red, HERPUD1.
Citation(0)
  • 基因名：
    HERPUD1
  • 货号：
    65049
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-HERPUD1 antibody (Cat#65049). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HERPUD1 antibody (Cat#65049, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HERPUD1 antibody (Cat#65049). HERPUD1 expression in wild-type (WT) and HERPUD1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HERPUD1 antibody (Cat#65049, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-HERPUD1 antibody (Cat#65049, 1:1,000), Top panel: wild-type (WT); Bottom panal: HERPUD1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HERPUD1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of HERPUD1 expression in C2C12 cells using anti-HERPUD1 antibody (Cat#65049, 1:1,000). Green, isotype control; red, HERPUD1.
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