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KD-Validated Anti-OTUB1 Mouse Monoclonal Antibody #63553

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Flow cytometric analysis of OTUB1 expression in HeLa cells using anti-OTUB1 antibody (Cat#63553, 1:2,000). Green, isotype control; red, OTUB1.

Western blotting analysis using anti-OTUB1 antibody (Cat#63553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-OTUB1 antibody (Cat#63553, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-OTUB1 antibody (Cat#63553). OTUB1 expression in wild type (WT) and OTUB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-OTUB1 antibody (Cat#63553, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Validation of OTUB1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-OTUB1 antibody (Cat#63553, 1:2,000) and analyzed using BD flow cytometer.

基因名：

OTUB1
货号：

63553
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-OTUB1 Mouse Monoclonal Antibody
Aliases	OTUB1; OTU Deubiquitinase, Ubiquitin Aldehyde Binding 1; OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 1; Ubiquitin-Specific-Processing Protease OTUB1; OTU Domain, Ubiquitin Aldehyde Binding 1; Deubiquitinating Enzyme OTUB1; Ubiquitin Thioesterase OTUB1; Otubain-1; FLJ20113; FLJ40710; OTB1; OTU1; Ubiquitin-Specific Protease Otubain 1; OTU-Domain Ubal-Binding 1; EC 3.4.19.12; EC 3.1.2; HSPC263; HOTU1
Background	Gene Name: OTUB1 NCBI Gene Entry: 55611 UniProt Entry: Q96FW1
Application Information	Molecular Weight: Predicted, 31 kDa; observed, 35 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB6485 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human OTUB1
Isotype	Mouse IgG2b
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of OTUB1 expression in HeLa cells using anti-OTUB1 antibody (Cat#63553, 1:2,000). Green, isotype control; red, OTUB1.
Western blotting analysis using anti-OTUB1 antibody (Cat#63553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-OTUB1 antibody (Cat#63553, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-OTUB1 antibody (Cat#63553). OTUB1 expression in wild type (WT) and OTUB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-OTUB1 antibody (Cat#63553, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of OTUB1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-OTUB1 antibody (Cat#63553, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

OTUB1
货号：

63553
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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