Flow cytometric analysis of pro Caspase 3 in HAP-1 cells using pro Caspase 3 antibody (Cat#68673, 1:2,000). Green, isotype control; red, pro Caspase 3.
Immunocytochemical staining of HAP-1 cells with pro Caspase 3 antibody (Cat#68673, 1:1,000). Nuclei were stained blue with DAPI; pro Caspase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-pro caspase 3 antibody (Cat#68673, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-pro Caspase 3 antibody (Cat#68673). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pro Caspase 3 antibody (Cat#68673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-pro Caspase 3 antibody (Cat#68673). pro Caspase 3 expression in wild type (WT) and pro Caspase 3 shRNA knockdown (KD) HAP1 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-pro Caspase 3 antibody (Cat#68673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of pro Caspase 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-pro Caspase 3 antibody (Cat#68673, 1:2,000) and analyzed using CytoFLEX.
